Cytokeratin 19 significance in normal and HPV infected oral mucosa

Suha A.H. Hind



The present retrospective study was performed on normal oral mucosa and infected oral mucosa with human papilloma virus (HPV).

With main objective to establish the expression of cytokeratin 19 in normal and HPV infected oral mucosa due to the virus effect.

Materials and Methods

This study involves a total of (34) cases, (24) sections from oral mucosa of 24 females were
infected with HPV virus, and (10) sections from normal mucosa. The expression of cytokeratin 19 was carried out on 4μm speci- men sections using Immuno histochemical staining of cytokeratin 19 antibody.
The staining demonstrated the expression intensity, percentage and localization.


Results show cytokeratin 19 was expressed in both normal and HPV infected oral mucosa, but the expression in infected mucosa was significantly different from that of normal one, and the intensity of the staining was more in the basal layer of infected mucosa than normal, in addition to that results show both nuclear and cytoplasmic expression and involves the whole layers of the infected mucosa.


HPV can infect oral mucosa and persistent HPV infection in the oral mucosa might increase the risk of developing oral cancer due to its effect on the differentiating host keratinocyte cells, so HPV infection is one of the contributing factors for OSCC.


Cytokeratin 19, Human papilloma virus (HPV).

Full Text:



- Dyavanagoudar S, Kale A, Bhat K, Hallikerimath S. Reverse transcriptase polymerase chain reaction study to evaluate dissemination of cancer cells into circulation after incision biopsy in oral squamous cell carci- noma. Indian J Dent Res 2008;19: 5-9.

- Moll R, Franke WW, Schiller DL. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982; 31:11–24.

- Sawaf MH, Ouhayoun JP, Forest N. Cytokeratin profiles in oral epithelia: a review and a new classifica- tion. J Biol Buccale. 1991;19:187–98.

- Chu PG, Weiss LM. Keratin expression in human tissues and neoplasms. Histopathology. 2002;40:403–39.

- Kirfel J, Magin TM, Reichelt J. Keratins: a structural scaffold with emerging functions. Cell Mol Life Sci. 2003;60:56–71.

- Ibrahim SO, Warnakulasuriya KA, Idris AM, Hirsch JM, Johnson NW, Johannessen AC. Expression of keratin 13, 14 and 19 in oral hyperplastic and dysplastic lesions from Sudanese and Swedish snuff-dippers: association with human papillomavirus infection. Anticancer Res. 1998; 18: 635-45.

- Schiffman M, Castle PE. Human Papillomavirus: epidemiology and public health . Arch Pathol Lab Med.

; 127: 930.

- Syrjanen S, Rautava J. Human papilloma virus infections in the oral mucosa. The Journal of the American DentalAssociation 2011;42:905-914.

- Gillson J, Mayrand M H, Franco ED, Isabel Rodrigues I, Walter SD . Human Papilloma Virus DNA versus papanicolaou screening tests for cervical cancer. New England Journal of Medicine 2007; 357:1579-1588.

- Harboe N, (2005). Cytokeratin 19 monoclonal mouse anti-human. Immunocytochemistry; 2005. p. 220.

- Schiller JT, Day PM, Kines RC. Current understanding of the mechanism of HPV infection. Gynecologic Oncology 2010; 118:12.

- Kellokoski, J, Syrjänen, S, Tosi, P, Cintorino M, Leoncini P, Syrjänen K. Cytokeratin pattern in normal and HPV infected oral mucosa in women with genital HPV infections. Journal of Oral Pathology & Medicine 2006; 20: 26–31.

- Greenblatt R.J. 2005: Human papillomaviruses: Diseases, diagnosis, and a possible vaccine. Clinical Mi- crobiology Newsletter 2005; 27: 139-145.

- Sinal SH, Woods CR . Human papillomavirus infections of the genital and respiratory tracts in young children. Seminars in pediatric infectious diseases 2005; 16: 306–16.

- D’Souza G, Kreimer AR, Viscidi R. Case control study of human papillomavirus and oropharyngeal can- cer. J Engl. Med. 2007; 356:1944-56.

- Ridge JA, Glisson BS, Lango MN, Wagman LD, Camphausen KA, Hoskins WJ. Head and Neck Tumors. Cancer management: A multidisciplinary approach. 11th ed. 2008. p. 170-73.

- Ganguly N, Parihar SP. Human papillomavirus E6 and E7 oncoproteins as risk factors for tumorigenesis. Journal of biosciences 2009; 34:113–123.

- Chaturvedi A, Maura L, Gillison F. Human papilloma virus and head and neck cancer. Epidemiology, Pathogenesis, and Prevention of Head and Neck Cancer. 1st ed. 2010. p. 231-36.

- Münger DA, Howley PM. Human papillomavirus immortalization and transformation functions. Virus research 2002; 89: 213–28.

- Kellokoski J, Syrjänen S, Tosi P, Cintorino M, Leoncini P, Syrjänen K.Cytokeratin pattern in normal and HPV infected oral mucosa in women with genital HPV infections. Journal of Oral Pathology & Medicine 2006; 20: 26-31.

- Saini R, Tang TH, Zain RB, Cheong SC, Musa KI, Saini D, Ismail AR, Abraham MT. Significant as- sociation of high-risk human papilloma virus (HPV) but not of p53 polymorphisms with oral squamous cell carcinomas in Malaysia. J Cancer Res Clin Oncol. 2011; 137: 311-20.

- Sugerman, P, Shillitoe E. The high risk human papillomaviruses and oral cancer: evidence for and against a causal relationship. Oral Diseases 1997; 3: 130–147.



  • There are currently no refbacks.


Copyright by Iraqi Dental Journal

ISSN (Print): 2307-4779 | ISSN (Online): 2411-9741