Assessment of some salivary biochemical parameters in cigarette smokers with chronic periodontitis

Mahmood Y. Gazy, Ahmed B. Mohiaddin, Ahmed Z. Al-Kasab


Background and objectives: Cigarette smoking is an important risk factor that has a clear strong association with the prevalence

and severity of chronic periodontitis (CP). Salivary biochemical parameters may be affected by both smoking and CP together.

Method: Eighty systematically healthy male, were included in this study. They were grouped based on their periodontal and smoking status. Unstimulated whole saliva (UWS) was collected from all subject. Salivary flow rate (FR) was measured during sample collection. Parameters such as salivary pH, total protein (TP), albumin (Alb), globulin (Glo), total fucose (TF), protein bound fucose (PBF) and C-reactive protein (CRP) were estimated.

Results: Salivary flow rate was not altered regarding to periodontal health status. Salivary pH was lower in smokers than in non- smokers, while it was not affected by periodontal health status. TP, Alb and Alb/Glo ratio were higher in CP patients. Saliva Glo and TF levels increased in both CP and smokers with CP, while salivary PBF level decreased in both CP and smokers with CP comparing to healthy control. The concentrations of these parameters did not affect by smoking status except for TP. Regarding CRP, in gen- eral, its level was higher in smokers than in non- smokers, while it was not affected by periodontal health status.

Conclusion: Both smoking and chronic periodontitis together affect some salivary biochemical parameters, thus the concentra- tions of these parameters could be used as indicators for periodontal disease progression and severity in smokers with CP. Both smoking and periodontal health status together should be taken in consideration when salivary composition is studied.

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- Williams R. (2008) Understanding and Managing Periodontal Diseases: A Notable Past, A Promising Fu- ture. J Periodont; 79 (8):1552-1559.

- Petersen P. and Ogawa H. (2005) Strengthening the prevention of periodontal disease: The WHO Ap- proach. J Periodontol; 76(12):2187-2193.

- Armitage G. and Robertson P. (2009) The Biology, Prevention, Diagnosis and Treat-

ment of

Periodontal Diseases. JADA; 140(1): 36s-43s.

- Genco R., Offenbacher S. and Beck S. (2002) Periodontal disease and cardiovascular disease; Epidemiol- ogy and possible mechanisms. JADA; 133(1):14-22.

- Kornman K. (2008) Mapping the Pathogenesis of Periodontitis: A New Look. J Periodont; 79 (8): 1560- 1568.

- Carranza F., Takie H. and Newman M. (2010) Text book of clinical Periodontology. 10th Edition .Phila- delphia: W.B. Saunders Company, USA.

- Curry-Chiu M. (2010) Tobacco Use and Periodontal Diseases: Background Information and Tools for Den- tal Hygiene Students. JCCC Honor Journals; 1(2):1s-11s.

- Reibel J. (2003) Tobaco and Oral Disease .Med Prince Pract; 12 (4) (suppl 1) : 22-23.

- Jette A., Feldman H., and Tennstedt S. (1993) Tobacco use: a modifiable risk factor for dental disease among the elderly. Am J Public Health; 83(9): 1271-1276.

- Mullaly B. (2004) The Influence of Tobacco Smoking on the Onset of Periodontitis in Young Persons. Bio Med Central; 2 (2) : 53-65.

- Bortold P., Ishikawa I. and De Deios N. (2003) Current trends in periodontal diagnosis, disease recogni- tion and management. Proceedings of the 5th Asian Pacific Society of Periodontology Meeting, Cebu , The Philippines, 1-2 December .

- Inoue H., Ono K., Masuda W., Moremoto Y.,Tanaka T., and Yokota M. (2008) Gender differenc in un- stimulated whole saliva flow rate and salivary gland sizes, Archive of Oral Biology; 51:1055- 1060.

- de Almeida P., Gregio A., Machado M., de Lima A. and Azevedo L.(2008) Saliva Composition and Func- tion :A Comprehensive review,The Journal of Contemporary Dental Practice; 9(3):72-80.

- Kaufman E. and Lamster I. (2002) The Diagnostic Application of Saliva –A Review. CROBM ;13 (2):197- 212.

- Lamster I. (2006) Antimicrobial mouthrinses and the management of periodontal diseases. JADA; 137(3):5s-9s.

- Sculley D. and Langley-Evans M. (2002) Salivary antioxidants and periodontal disease status. The Nu- tritional Society; 61:137- 141.

- Aurer A., Jorgic-Srdjak K., ,Plancak D.,Stavljenic-Rukkavina A. and Aurer-Kozelj J. (2005) .Proinflama- tory factors in saliva as possible Markers for periodontal disease. Coll.Antropol;29(2):435-439.

- Page R., and Eke L. (2007) Case definitions for use in population-based surveillance of periodontitis. J periodontal; 78(7) (suppl.):1267-1399.

- Rao T. (2007) Text book of Periodontology. 2nd ed, All India Publishers, New Dehli, India.

- Varma B. and Nayak R. (2009) Clinical Periodontology. Arya Publishing Hause, New Delhi,India.

- Flink H. (2005) Unstimulated Human Saliva Flow Rate in Relation to Hyposalivation and Dental Caries, Ph D Thesis, Institute of Odontology , Karoliniska Institute, Stockholm, Sweden.

- Shitty P. and Pattabiraman T. (2004) Salivary glycoprotein as indicators of oral disease . Indian Journal of Clinical Biochemistry; 19(1): 97-101.

- Sialomitrics. Saliva collection hand book.2010.[on line] Available in www. sialometrics. com. Last viewed in 25th Mar 2011.

- Rantonen P. (2003) Salivary flow and composition in healthy and disease adults. M.Sc. Thesis, Faculity of medicine, Helsinki University, Helsinki, Finland.

- Tietz N. (1999) Text book of clinical chemistry. 3rd Edition, E.R. Ashwood: W.B. Saunders,USA.

- Al-Sarrag N. (2005) α-L-fucose as a possible diagnostic marker with other related parameters in thyroid dysfunction patients. Ph D thesis, College of Education, Baghdad University, Iraq.

- Heintze U. (1984) Secretion rate, buffer effect and number of lactobacilli and Streptococcus mutans of whole saliva of cigarette smokers and nonsmokers. Scand J Dent Res; 92 (4): 294-301.

- Parvinen T. (1984) Stimulated salivary flow rate, pH and lactobacillus and yeast concentrations in non- smokers and smokers. Scand J Dent Res; 92(4): 315-318.

- Bezerra Junior A.,Pallos D., Cortelli J., Coury Saraceni C. and Queiroz C. (2010) Evaluation of organic and inorganic compounds in the saliva of patients with chronic periodontal diseases, Rev.odonto scienc; 25(3) : 234-238.

- Crow H. and Ship J. (1995) Are gingival and periodontal conditions related to salivary gland flow rates in healthy individuals? JADA; 126(11): 1514-1520.

- Shaila M. (2006) Salivary protein concentration ,Buffer capacity and pH estimation , A Comparative study among young and elderly Subjects: both normal and with gingivitis and periodontitis . M.Sc. Thesis, Rajiv Gandhi University of health science, Karnataka, Bangalore, India .

- Sculley D. and Langley-Evans C. (2003) Periodontal disease is associated with lower antioxidant capacity in whole saliva and evidence of increased protein oxidation. Clinical Science; 105:167-172.

- Aziz A. and Askari S.(2008) Comparison of Unstimulated Saliva Secretion Between Heavy Smokers and Non smokers Subject. Journal of Guilan University of Medical Sciences; 69:11-16.

- Gonzalez O., Martinez B. and Villarroel-Dorrego M. ( 2009) Salivary pH alteration in smoker patients with periodontal disease. AVANCES; 21(2):71-75.

- Watanabe T, Soeda W, Kobayashi K and Nagao M. The pH value changes in the periodontal pockets. J Bull Tokyo Med Dent Univ 1996; 43(4): 67-73.

- Bosshardt D. and Lang P. (2005) The junctional epithelium:from health to disease. JDR; 84:9-20.

- Bergstrom J. and Bostrom L. (2001) Tobacco smoking and periodontal hemorrhagic responsiveness.J Clin ; 28: 680-685.

- Nagler M. (2007) Altered salivary profile in heavy smokers and it is possible connection to oral cancer. The International Journals of Biological Markers; 22:274-280.

- Nishida N.,Yamamoto Y.,Tanaka M.,Hayashi N.,Nakayama K., and Morimoto K..(2004) Effect of Active Smoking on Salivary Biomarkers Related to Periodontitis. Proceding to 53rd, Annal Meeting of Japanese As- sociation for Dental research , Okayama, Japan, 27-28 November .



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