Antibacterial Effect of Green Tea Extract Epigallocatechin Gallate against Enterococcus faecalis as Intracanal Medicament (An in vitro study)

Zena A Ahmad, Manaf B Ahmed, Wiaam M.O. AL-Ashou

Abstract


Abstract

Objectives: The aim of this study was to assess the antibacterial efficiency of various concentrations of green tea extract Epigallocatechin Gallate (EGCG) as intracanal medicament and compared it with the effect of calcium hydroxide paste on Enterococcus faecalis. Material and methods: Intracanal medicament was prepared by mixing powder of EGCG extract with glycerol material. Forty human single rooted teeth were prepared, sterilized, cultured with E. faecalis and incubated for 24 hours. Specimens were taken from root canal using file and CFU of bacterial colonies were measured. Teeth were classified into 4 groups: negative control group with no intracanal medicament, positive control group with calcium hydroxide paste intracanal medicament, and three tested groups with 2%, 3%, and 4% of prepared EGCG intracanal medicaments. After 3days; the intracanal medicaments were removed, specimens were taken and bacterial colonies were measured by CFU to assess the antibacterial activity in each group. Results: All tested groups showed significant antibacterial effect against E.fecalis. EGCG group at 2% showed the least antibacterial effect. While both 3% and 4% EGCG groups showed high antibacterial effect which was similar to that of Ca (OH)2. Conclusion: Green tea catechine (EGCG) can be used effectively as intracanal medicament, specifically at concentration of 3% and 4%.

 


Keywords


Keywords: Epigallocatechin Gallate; Intracanal medicament; Enterococcus faecalis; Green tea; Enterococci; Endodontic infections

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References


Divia AR, Nair MG, Varughese JM, Kurien S. A comparative evaluation of Morinda citrifolia, green tea polyphenols, and Triphala with 5% sodium hypochlorite as an endodontic irrigant against Enterococcus faecalis: An in vitro study. Dent Res J (Isfahan). 2018;15(2):117-122.

Jose J, Shoba K, Aman S, Tomy N, Christie S. Comparative Evaluation of Antimicrobial Activity of Green Tea Extract, Garlic Extract, Neem Leaf Extract and Sodium Hypochlorite as Root Canal Irrigants against E. Faecalis and C. albicans. An In Vitro Study. Int J Curr Microbiol App Sci., 2015; 4(10): 384-391.

Mohammadi Z, Jafarzadeh H, Shalavi S, Kinoshita JI. Unusual Root Canal Irrigation Solutions. J Contemp Dent Pract. 2017;18(5):415-420.

Lana PEP, Scelza MFZ, Silva LE, Mattos-Guaraldi AL, Hirata- Ju´nior R. Antimicrobial activity of calcium hydroxide pastes on Enterococcus faecalis cultivated in root canal systems. Braz Dent J. 2009; 20:32e6

Attia DA, Farag AM, Afifi IK, Darrag AM. Antimicrobial effect of different intracanal medications on various Microorganisms. Tanta Dental Journal. 2015;12(1): 41-47.

Pujar M, Makandar SD. Herbal Usage In Endodontics- A Review. Int J of Contemporary Dentistry. 2011; 1(2): 34-36.

Oncag O, Cogulu D, Uzel A, Sorkun K. Efficacy of propolis as an intracanal medicament against Enterococcus faecalis. Gen Dent. 2006;54(5):319-322.

Prabhakar J, Senthilkumar M, Priya MS, Mahalakshmi K, Sehgal PK, Sukumaran VG. Evaluation of antimicrobial efficacy of herbal alternatives (Triphala and green tea polyphenols), MTAD, and 5% sodium hypochlorite against Enterococcus faecalis biofilm formed on tooth substrate: an in vitro study. J Endod. 2010;36(1):83-86. doi: 10.1016/j.joen.2009.09.040.

Ferrazzano GF, Amato I, Ingenito A, Zarrelli A, Pinto G, Pollio A. Plant polyphenols and their anti-cariogenic properties: a review. Molecules. 2011;16(2):1486-1507.

https://orbitbiotech.com/how-to-calculate-cfu-colony-forming-unit-cfu-colony-forming-unit-cfu-ml-cfu-g-cell-count-microbial-counting/

Martina LP, Ebenezar AV, Ghani MF, Narayanan A, Sundaram M, Mohan AG. An in vitro comparative antibacterial study of different concentrations of green tea extracts and 2% chlorhexidine on Enterococcus faecalis. Saudi Endod J 2013; 3:120-4.

Sathorn C, Parashos P, Messer H. Australian endodontists' perceptions of single and multiple visit root canal treatment. Int Endod J. 2009; 42:811e8.

Waltimo TM, Haapasalo M, Zehnder M, Meyer J. Clinical aspects related to endodontic yeast infections. Endod Top. 2004; 9:66e78

Tang G, Samaranayake LP, Yip Hk. Molecular evaluation of residual endodontic microorganisms after instrumentation, irrigation and medication with either calcium hydroxide or septomixine. Oral Dis 2004; 10:389e97.

Souza-Filho FJ, Soares Ade J, Vianna ME, Zaia AA, Ferraz CC, Gomes BP. Antimicrobial

effect and pH of chlorhexidine gel and calcium hydroxide alone and associated with other materials. Braz Dent J. 2008; 19:28e33.

Taylor PW, Hamilton Miller JM, Stapleton PD. Antimicrobial properties of green tea catechins. Food Sci Technol Bull 2005; 2:71 81.

Sun Y, Hung WC, Chen FY, Lee CC, Huang HW. Interaction of tea catechins epigallocatechin gallate with lipid bilayers. Biophys J 2009; 96:1026 35.

Gradisar H, Pristovsek P, Plaper A, Jerala R. Green tea catechins inhibit bacterial DNA gyrase by interaction with its ATP binding site. J Med Chem 2007; 50:264 71.

Shabbir J, Khurshid Z, Qazi F, Sarwar H, Afaq H, Salman S, Adanir N. Effect of Different Host-Related Factors on Postoperative Endodontic Pain in Necrotic Teeth Dressed with Interappointment Intracanal Medicaments: A Multicomparison Study. Eur J Dent. 2021;15(1):152-157. doi: 10.1055/s-0040-1721909.

Yaghmoor RB, Platt JA, Spolnik KJ, Chu TMG, Yassen GH. Effect of Hydrogel-Based Antibiotic Intracanal Medicaments on Push-Out Bond Strength. Eur J Dent. 2020 Oct;14(4):575-583. doi: 10.1055/s-0040-1713956.

Pribadi N, Budiarti D, Kurniawan HJ, Widjiastuti I. The NF-kB and Collagen Type 1 Expression in Dental Pulp after Treated Calcium Hydroxide Combined with Propolis. Eur J Dent. 2021 Feb;15(1):122-126. doi: 10.1055/s-0040-1716319.

De Souza V, Zaniboni JF, Escalante-Otárola WG, Matsumoto MA, Bighetti CC, Kuga MC. Effects of the incorporation of alpha-tocopherol as antioxidant on biological and physicochemical properties of calcium hydroxide associated with bioactive vehicle. Eur J Gen Dent 2020;9:157-62.

Sarraf P, Assadi A, Kiomarsi N, Mohammadian F, Assadi A, Shamshiri AR. Effect of Intracanal Medicaments on Pull-Out Bond Strength of Fiber Post Cemented with a Self-Adhesive System. Eur J Dent. 2019 Jul;13(3):420-425. doi: 10.1055/s-0039-1694313.

Gougousis K, Giannakoulas DG, Taraslia V, Agrafioti A, Anastasiadou E, Kontakiotis EG. Number of Dental Stem Cells on Root Canal Dentin after Application of Triple Antibiotic Paste or Calcium Hydroxide: An In Vitro Study. Eur J Dent. 2019 May;13(2):161-165. doi: 10.1055/s-0039-1688733.

Jindal V, Monga S, Jindal D. Comparative Evaluation of the Interappointment Pain by Using Different Intracanal Medicaments—An In Vivo Study. Dent J Adv Stud 2019; 7:19–22.

Mustafa M, Alaajam WH, Azeim AA, Alfayi NA, Alqobty RM, Alghannam S. Diffusion of calcium hydroxide through dentinal tubules of retreated root canals: An in vitro study. Eur J Dent 2018; 12:386-92.

Mehta S, Verma P, Tikku AP, Chandra A, Bains R, Banerjee G. Comparative evaluation of antimicrobial efficacy of triple antibiotic paste, calcium hydroxide, and a proton pump inhibitor against resistant root canal pathogens. Eur J Dent 2017; 11:53-7.




DOI: http://dx.doi.org/10.46466/idj.v45i1.277

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