The Histopathological Effect of Local Injection of Fucose on Bone Fracture :An Experimental Study on Rabbits

Chenar A. Mohammad, Amal Hana Aziz, Aveen Ajeel

Abstract


Aim: To evaluate the effect of sulcular injection of 50μl of 150mM fucose on rabbits with bone trauma.
Background: α-L-Fucose is a methyl pentose sugar similar to L-galactose except for the loss of alcohol group on
carbon number 6.It is found in human serum bound to proteins by covalent bonding and it is a member of a large
group of compounds known as covalent bond membranes .
Materials and Methods: The present study was carried out on 65 male rabbits, bone trauma was introduced into the mid- labial
areas of facial plate of the alveolar bone of lower right central incisor for all rabbits . The rabbits had been left for 7 days after
induction of bone trauma, then 5 rabbits were sacrificed and considered as a base line traumatic group . The remaining 60 rabbits
were divided randomly into two 2 main groups, first group consist of 30 rabbits and were injected locally with 50μl of 150mM fucose
solution into the bottom of gingival sulcus at the mid-labial area of lower right central incisor and were considered as fucose
injected group and were divided randomly into six subgroups(each subgroup consist of 5 rabbits) , then periodontal tissue biopsy
was collected from injected group at time intervals of 1, 3 ,7 ,14, , 21 , and 28 days after fucose injection , while the second group
consist of 30 rabbits with bone trauma only and were not received any fucose injection and considered as non injected group and
were divided randomly into six subgroups (each subgroup consist of 5 rabbits), sacrificed at time intervals of 8,10,14,21,28 ,and
35 days after induction of bone fracture.
Results: The results showed that fucose injection enhanced bone regeneration and healing of traumatic bone in short duration
of time, 1 day after sulucular injection of fucose (8 days after induction of bone fracture).
Conclusion: α-L- Fucose can be used as a bone stimulating agent which stimulate and accelerate new bone formation, so fucose
can be used in the treatment of alveolar bone defect in periodontitis patient


Keywords


α-L-Fucose; periodontal biopsy; sulcular injection

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References


Rosato FE, Seltzer M, Mullen J Rosato E F.Serum fucose in

the diagnosis of breast cancer. Can 1971; 28(6):1575-1579.

Becker DJ and Lowe JB .Fucose :biosynthesis and biological

function in mammals. Glycobio 2003; 13(7): 41-53.

Moriwaki k and Miyoshi E. Fucosylation and gasterointestinal

cancer. Word J Heptaol 2010; 2 (4) :151-61.

Mondoa MD, E.n bvcmil I, and Kitei M. Sugars That Heal

: The New Healing Science of Glyconutrients. Ballantine

Publishing.biochemistry 2001; 100: 1387-98.

Abbas LB and Ahmed SA . Serum total α-L-fucose and

related parameters in breast cancer as tumor marker 2011;

(1).

AlanW. Fucose.e-Newsletter.American International

Association. 2006.

Park D, Ryu KS,Choi JK and ParkC .Characterization and

role of fucose mutarotase in mammalian cells .Glycobio

;17(9):955-962.

Shetty PK, Pattabiraman TN. Salivary glycoprotein as

indicators of oral diseases. Indi J Clini Bioch 2004;19(1)

: 97-101.

Shah M, Telang S, Ravel G, Shah P and Patel PS.Serum

fucosylation changes in oral and precancerous conditions.

Cancer 2008 ;113 (2):336-346.

Chitra S and Shamala Davi CSS .Effect of vitamin E on

protein bound carbohydrate complexesin radiation treated

oral squamous cell carcinoma patients. Indi J Clini Bioch

; 23(3):92-49.

Mahmood Y CH. ASSESSMENT OF SOME SALIVARY

BIOCHEMICAL COMPOSITION IN CIGARETTE

SMOKER WITH CHRONIC PERIODONTITIS. 2011

. Master thesis, College of Dentistry, Hawler Medical

University, Iraq.

Wsoo MA. Biochemical Studies on Salivary α-L-Fucose

and its related parameters in periodontitis 2012.

Master thesis.College of Science . Koya University.Iraq

Katsuhisa N ,Eji M, Gu J, Cong-Xiao G, Susumu N and

Takatoshi K et al . Relationship between elevated FX

expression and increased production of GDP-L-Fucose,

a common donar substrate for fucosylation in human

hepatocellular carcinoma and hepatocell lines . Can Res

; 63(19): 6282-6289.

Murray RK , Granner DK and Rodwell VW

(2006a). Harpers illustrated Biochemistry 27th ed .

Singapore : McGraw-Hill 2006; Pp. 51,62, 65,6869 ,89,2

Cardarelli PM, Lomis MCM, Preston B, Black A, Passmore

D et al..In vitro and in vivo characterization of MDX-

for therapy of Malignant Lymphoma –Clinical Can

Res 2009 ; 15:3376 .

Etzioni A , Tonetti M, Vestweber D and Marqua M .Fucose

supplementation in leukocyte deficiency type II .Blood

; 95(11):3641.

Omer RM. Effect of α- L-fucose on Rabbit tongue muscle

Phd thesis , College of Dentistry, Hawler

Medical University,Iraq.

Mohammad AM..The effect of intracrevicular gingival

injection of α- L-fucose. Biochemical,immunological and

biochemical study on rabbits 2013 .Phd thesis , College

of Dentistry, Hawler Medical University,Iraq.

Hedenqqvist P. Anasthesia and analgesia for surgery in

rabbits and rats: A comparison of the effects of different

comounds 2008.Ph.D thesis,stockholm,sweden.

Hussien SS. Hitological effects of ciprofloxacin and

ceftriaxone on the healing process of the alveolar bone

Master thesis .College of Dentistry, Hawler Medical

University




DOI: http://dx.doi.org/10.26477/idj.v37i2.48

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