The Effect of Nd:YAG Laser and Casein- Phosphor-Peptide-Amorphous Calcium Phosphate with Fluoride on Dental Enamel De-mineralization: In vitro study

Hiba Issam Abdulahhab, Ali Shukur Mahmood

Abstract


The aim of the study was to evaluate a new treatment modality to decrease dental enamel demineralization via combing the effect
of pulsed Neodymium Yttrium Garnet (Nd:YAG) laser (1064 nm) and Re-mineralizing paste(CPP-ACP containing sodium fluoride)
regarding the tooth pulp chamber temperature elevation, calcium concentration dissolute from enamel surfaces and the dental
enamel absorption of methylene blue stain by assessing (R red ,G green ,B blue)values. Sixty human permanent premolars were
used for measuring calcium concentration dissolution and (R,G,B)s values. They randomly divided into six groups: the negative
control group (A) received no treatment, and the positive control group (B) where the re-mineralizing paste was applied without
laser irradiation. For the groups (C), (D), (E) and (F) the (1064 nm) pulsed Nd:YAG laser was used in two dose settings. The lower
laser dose setting at (60 mJ) for the C and D groups,and the higher laser dose setting at (170mJ)for E and F groups respectively.
Groups D and E in addition to laser irradiation the Re-mineralizing paste was applied.
The atomic absorption photometry test results showed that groups C, and D were statistically different compared to the groups
A and B (p<0.001) in the reduction of calcium dissolution, Whereas Groups E and F shown non-significant reduction in calcium
dissolution compared to control groups.
Twenty teeth were used to assess the temperature elevation during irradiation using a K-type thermocouple at 37 ± 0.5 ̊C, divided
for ten teeth for each irradiation settings. The maximum tempers of temperature test showed that the maximum temperature
increases for group E was very high significant difference (p<0.05) over that of groups C.
The (R,G,B)s value test showed that groups C and D having lower affinity to absorb methylene blue stain, and they are statically
significant different compared to the groups A and B .while groups E and F showing a higher affinity to absorb methylene blue stain
compared to control groups.


Full Text:

PDF

References


Lima YBO, Cury JA. Seasonal variation of fluoride intake

by children in a subtropical region. Caries Res. J 2003

;37:335– 338.

Shen P, Cai F, Nowiciki A, Vincent J, Reynolds EC.

Remineralization of enamel sub surface lesions by sugar

free chewing gum containing casein phosphopeptideamorphous

calcium phosphate. Dent Res J.2001 ;80:

–70.

Reynolds EC. Remineralization of enamel subsurface

lesions by casein phosphopeptide-stabilized calcium

phosphate solutions. Dent Res.J 1997; 76(9):1587-95.

Reynolds EC, Cai F, Cochrane NJ, Shen P, Walker

GD, Morgan MV, Reynolds C. Fluoride and casein

phosphopeptide-amorphous calcium phosphate.Dent

Res.J 2008 ;87(4):344-8.

Hamba H, Nikaido T, Inoue G, Sadr A, Tagami J: Effects of

CPP-ACP with sodium fluoride on inhibition of bovine

enamel demineralization: A quantitative assessment using

micro-computed tomography. Dent J 2011; 39:405–413.

Stern, R.H., Sognnaes, R.F. & Goodman F. Laser effect on in

vitro permeability and solubility. Journal of the American

Dental Association, 1966;7(3) 4:838-43.

Korytnicki D, Mayer MP, Daronch M, Singer Jda M, Grande

rH. Effects of Nd:YAG laser on enamel microhardness

and dental plaque composition: an in situ study.Photomed

Laser Surg J 2006; 24:59-63.

Anderson J, Ellis R, Blankenau R, Beiraghi S, Westerman

G. Caries resistance in enamel by laser irradiation and topical fluoride treatment. J Clin Laser Med Surg

(2000);18:33-36.

Westerman G, Flaitz CM Powell G, Hicks M, Enamel caries

initiation and progression after argon laser irradiation:

in vitro argon laser systems comparison. J Clin Laser Med

Surg (2002); 20:257-262.

Featherstone JD, Nelson DG. Laser effects on dental hard

tissues. Adv Dent J res 1987; 1:21-26.

Zach L, Cohen G. Pulp response to externally applied

heat. Oral Surg Oral Med Oral Pathol J. 1965; 19:515–30.

Mohammed Abbood Al-maliky, Ali Shukur Mahmood,

Christoph Kurzmann1, Markus Laky, Alexander Franz,

Andreas Moritz, The effects of CO2 laser with or without

nano-hydroxyapatite paste in the occlusion of dentinal

tubules. Hidawi J 2014;798732: 5.

J.D. Featherstone and B.E. Rodgers,Effects of acetic ,lactic

and other organic acids on the formation of the artificial

carious lesion Caries J Res.J 1981 ;15: 377.

Featherstone JD. Caries detection and prevention with

laser energy. Dent Clin North AmJ 2000; 44:955-69.,14-

Tagomori S, Iwase T. Ultrastructural change of enamel

exposed to a normal pulsed Nd-YAG Laser. Caries ResJ

; 29:513-20.

Majori M, Manzon L, Pane S, Bedini r. Effects of Nd:YAG

laser on dental enamel. J Appl Biomater Biomech J 2005;

:128-133.

Bedini r, Manzon L, Fratto G, Pecci r. Microhardness

and morphological changes induced by Nd:YAG laser

on dental enamel: an in vitro study. Ann ist Super Sanita

; 46:168-172.

S.westland J,et al. Colour assessment in dentistry JAnnas

of BMVA 2007;4:1-10.

Meller C, et al. Anew in vivo method for measuring caries

activity with colour meter. J Clin Oral Investigation.

;10(2):140-144.

Zhao Shi-min ,Wang Jun .De-/RE-minerlization in

deciduous teeth assessed by color value analysis of digital

photographs .Shanghai J Stomatology, 2011;20(5)527-530.

A.A.Kunin ,etal. Coparative Analysis Of Effect Of Different

Tooth Paste On Caries Resistance Of Dental Enamel .J.

Clinical dentistry 2005;4.




DOI: http://dx.doi.org/10.26477/idj.v37i3.61

Refbacks

  • There are currently no refbacks.