The Effect of Nd:YAG Laser and Casein- Phosphor-Peptide-Amorphous Calcium Phosphate with Fluoride on Dental Enamel De-mineralization: In vitro study
Abstract
The aim of the study was to evaluate a new treatment modality to decrease dental enamel demineralization via combing the effect
of pulsed Neodymium Yttrium Garnet (Nd:YAG) laser (1064 nm) and Re-mineralizing paste(CPP-ACP containing sodium fluoride)
regarding the tooth pulp chamber temperature elevation, calcium concentration dissolute from enamel surfaces and the dental
enamel absorption of methylene blue stain by assessing (R red ,G green ,B blue)values. Sixty human permanent premolars were
used for measuring calcium concentration dissolution and (R,G,B)s values. They randomly divided into six groups: the negative
control group (A) received no treatment, and the positive control group (B) where the re-mineralizing paste was applied without
laser irradiation. For the groups (C), (D), (E) and (F) the (1064 nm) pulsed Nd:YAG laser was used in two dose settings. The lower
laser dose setting at (60 mJ) for the C and D groups,and the higher laser dose setting at (170mJ)for E and F groups respectively.
Groups D and E in addition to laser irradiation the Re-mineralizing paste was applied.
The atomic absorption photometry test results showed that groups C, and D were statistically different compared to the groups
A and B (p<0.001) in the reduction of calcium dissolution, Whereas Groups E and F shown non-significant reduction in calcium
dissolution compared to control groups.
Twenty teeth were used to assess the temperature elevation during irradiation using a K-type thermocouple at 37 ± 0.5 ̊C, divided
for ten teeth for each irradiation settings. The maximum tempers of temperature test showed that the maximum temperature
increases for group E was very high significant difference (p<0.05) over that of groups C.
The (R,G,B)s value test showed that groups C and D having lower affinity to absorb methylene blue stain, and they are statically
significant different compared to the groups A and B .while groups E and F showing a higher affinity to absorb methylene blue stain
compared to control groups.
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PDFReferences
Lima YBO, Cury JA. Seasonal variation of fluoride intake
by children in a subtropical region. Caries Res. J 2003
;37:335– 338.
Shen P, Cai F, Nowiciki A, Vincent J, Reynolds EC.
Remineralization of enamel sub surface lesions by sugar
free chewing gum containing casein phosphopeptideamorphous
calcium phosphate. Dent Res J.2001 ;80:
–70.
Reynolds EC. Remineralization of enamel subsurface
lesions by casein phosphopeptide-stabilized calcium
phosphate solutions. Dent Res.J 1997; 76(9):1587-95.
Reynolds EC, Cai F, Cochrane NJ, Shen P, Walker
GD, Morgan MV, Reynolds C. Fluoride and casein
phosphopeptide-amorphous calcium phosphate.Dent
Res.J 2008 ;87(4):344-8.
Hamba H, Nikaido T, Inoue G, Sadr A, Tagami J: Effects of
CPP-ACP with sodium fluoride on inhibition of bovine
enamel demineralization: A quantitative assessment using
micro-computed tomography. Dent J 2011; 39:405–413.
Stern, R.H., Sognnaes, R.F. & Goodman F. Laser effect on in
vitro permeability and solubility. Journal of the American
Dental Association, 1966;7(3) 4:838-43.
Korytnicki D, Mayer MP, Daronch M, Singer Jda M, Grande
rH. Effects of Nd:YAG laser on enamel microhardness
and dental plaque composition: an in situ study.Photomed
Laser Surg J 2006; 24:59-63.
Anderson J, Ellis R, Blankenau R, Beiraghi S, Westerman
G. Caries resistance in enamel by laser irradiation and topical fluoride treatment. J Clin Laser Med Surg
(2000);18:33-36.
Westerman G, Flaitz CM Powell G, Hicks M, Enamel caries
initiation and progression after argon laser irradiation:
in vitro argon laser systems comparison. J Clin Laser Med
Surg (2002); 20:257-262.
Featherstone JD, Nelson DG. Laser effects on dental hard
tissues. Adv Dent J res 1987; 1:21-26.
Zach L, Cohen G. Pulp response to externally applied
heat. Oral Surg Oral Med Oral Pathol J. 1965; 19:515–30.
Mohammed Abbood Al-maliky, Ali Shukur Mahmood,
Christoph Kurzmann1, Markus Laky, Alexander Franz,
Andreas Moritz, The effects of CO2 laser with or without
nano-hydroxyapatite paste in the occlusion of dentinal
tubules. Hidawi J 2014;798732: 5.
J.D. Featherstone and B.E. Rodgers,Effects of acetic ,lactic
and other organic acids on the formation of the artificial
carious lesion Caries J Res.J 1981 ;15: 377.
Featherstone JD. Caries detection and prevention with
laser energy. Dent Clin North AmJ 2000; 44:955-69.,14-
Tagomori S, Iwase T. Ultrastructural change of enamel
exposed to a normal pulsed Nd-YAG Laser. Caries ResJ
; 29:513-20.
Majori M, Manzon L, Pane S, Bedini r. Effects of Nd:YAG
laser on dental enamel. J Appl Biomater Biomech J 2005;
:128-133.
Bedini r, Manzon L, Fratto G, Pecci r. Microhardness
and morphological changes induced by Nd:YAG laser
on dental enamel: an in vitro study. Ann ist Super Sanita
; 46:168-172.
S.westland J,et al. Colour assessment in dentistry JAnnas
of BMVA 2007;4:1-10.
Meller C, et al. Anew in vivo method for measuring caries
activity with colour meter. J Clin Oral Investigation.
;10(2):140-144.
Zhao Shi-min ,Wang Jun .De-/RE-minerlization in
deciduous teeth assessed by color value analysis of digital
photographs .Shanghai J Stomatology, 2011;20(5)527-530.
A.A.Kunin ,etal. Coparative Analysis Of Effect Of Different
Tooth Paste On Caries Resistance Of Dental Enamel .J.
Clinical dentistry 2005;4.
DOI: http://dx.doi.org/10.26477/idj.v37i3.61
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