The effects of polishing the fitting surface of acrylic base retainers on Methichillin resistance staphylococcus aureus; a laboratory study

Dheaa Al Groosh, Jonathan Pratten, Nigel Hunt


This in vitro study evaluated the effects of polishing the fitting surface of two acrylic based matetrials on the surface characteristics and biofilm formation of Methicillin Resistance Staphylococcus aureus (MRSA) biofilms. A simulated mouth model (Constant Depth Film Fermenter) was used to produce biofilms on autopolymerised and heat cure acrylic substrata. Surface parameters included surface roughness, hydrophobicity and surface free energy was evaluated after using the conventional polishing procedure to samples simulating the clinical situation. The results showed that MRSA has successfully adhered and grown in all samples with a similar pattern without a significant difference between the recovered viable bacterial counts. The rough surface provide protect- ed niche for MRSA against the dislodgment forces. In conclusion, the finding of suggested that polishing the fitting surface of acrylic base materials may facilitate MRSA biofilm removal. 


MRSA Biofilm, Hawley retainer, surface roughness

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