The effects of polishing the fitting surface of acrylic base retainers on Methichillin resistance staphylococcus aureus; a laboratory study

Dheaa Al Groosh, Jonathan Pratten, Nigel Hunt

Abstract


This in vitro study evaluated the effects of polishing the fitting surface of two acrylic based matetrials on the surface characteristics and biofilm formation of Methicillin Resistance Staphylococcus aureus (MRSA) biofilms. A simulated mouth model (Constant Depth Film Fermenter) was used to produce biofilms on autopolymerised and heat cure acrylic substrata. Surface parameters included surface roughness, hydrophobicity and surface free energy was evaluated after using the conventional polishing procedure to samples simulating the clinical situation. The results showed that MRSA has successfully adhered and grown in all samples with a similar pattern without a significant difference between the recovered viable bacterial counts. The rough surface provide protect- ed niche for MRSA against the dislodgment forces. In conclusion, the finding of suggested that polishing the fitting surface of acrylic base materials may facilitate MRSA biofilm removal. 


Keywords


MRSA Biofilm, Hawley retainer, surface roughness

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References


Schenkein HA, Burmeister JA, Koertge TE, Brooks CN, Best AM, Moore LV, et al. The influence of race and gender on periodontal microflora. Journal of periodontology. 1993 Apr;64)4(:292-6. PubMed PMID: 8387107.

Wade WG. The oral microbiome in health and disease. Pharmacological research : the official journal of the Italian Pharmacological Society. 2013 Mar;69)1(:137-43. PubMed PMID: 23201354.

Todar K. Pathogenic E. coli. Online textbook of bacteriology 2007.

Socransky SS, Haffajee AD. Implications of periodontal microbiology for the treatment of periodontal infections. Compendium. 1994 )18(:S684-5, 8-93; quiz S714-7. PubMed PMID: 8039206.

Sbordone L, Bortolaia C. Oral microbial biofilms and plaque-related diseases: microbial communities and their role in the shift from oral health to disease. Clinical oral investigations. 2003 Dec;7)4(:181-8. PubMed PMID: 14598129.

Mylonakis E, Calderwood SB. Infective endocarditis in adults. The New England journal of medicine. 2001 Nov 1;345)18(:1318-30. PubMed PMID: 11794152.

Schiff E, Pick N, Oliven A, Odeh M. Multiple liver abscesses after dental treatment. Journal of clinical gastroenterology. 2003 Apr;36)4(:369-71. PubMed PMID: 12642750.

Marques da Silva R, Caugant DA, Josefsen R, Tronstad L, Olsen I. Characterization of Streptococcus constellatus strains recovered from a brain abscess and periodontal pockets in an immunocompromised patient. Journal of periodontology. 2004 Dec;75)12(:1720-3. PubMed PMID: 15732877.

Ahn J, Chen CY, Hayes RB. Oral microbiome and oral and gastrointestinal cancer risk. Cancer causes & control : CCC. 2012 Mar;23)3(:399-404. PubMed PMID: 22271008.

Farrell JJ, Zhang L, Zhou H, Chia D, Elashoff D, Akin D, et al. Variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer. Gut. 2012 Apr;61)4(:582-8. PubMed PMID: 21994333.

Kolenbrander PE, London J. Adhere today, here tomorrow: oral bacterial adherence. Journal of bacteriology. 1993 Jun;175)11(:3247-52. PubMed PMID: 8501028. Pubmed Central PMCID: 204720.

Bos R, van der Mei HC, Busscher HJ. Physico-chemistry of initial microbial adhesive interactions--its mechanisms and methods for study. FEMS microbiology reviews. 1999 Apr;23)2(:179-230. PubMed PMID: 10234844.

Kuhar M, Funduk N. Effects of polishing techniques on the surface roughness of acrylic denture base resins. The Journal of prosthetic dentistry. 2005 Jan;93)1(:76-85. PubMed PMID: 15624002.

Al Groosh D, Roudsari GB, Moles DR, Ready D, Noar JH, Pratten J. The prevalence of opportunistic pathogens associated with intraoral implants. Letters in applied microbiology. 2011 May;52)5(:501-5. PubMed PMID: 21332760.

Goncalves TS, Spohr AM, de Souza RM, Macedo de Menezes L. Surface roughness of auto polymerized acrylic resin according to different manipulation and polishing

methods: an in situ evaluation. The Angle orthodontist.

Sep;78)5(:931-4. PubMed PMID: 18298208.

Perni S, Prokopovich P, Piccirillo C, Pratten J, Parkin IP, Wilson M. Toluidine blue-containing polymers exhibit potent bactericidal activity when irradiated with red laser light. Journal of Materials Chemistry. 2009;19)18(:2715-

Pratten J. Growing oral biofilms in a constant depth film

fermentor )CDFF(. Current protocols in microbiology.

Aug;Chapter 1:Unit 1B 5. PubMed PMID: 18770599.

Morgan TD, Wilson M. The effects of surface roughness and type of denture acrylic on biofilm formation by Streptococcus oralis in a constant depth film fermentor. Journal of applied microbiology. 2001 Jul;91)1(:47-53.

PubMed PMID: 11442713.

Bollen CM, Lambrechts P, Quirynen M. Comparison of

surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature. Dental materials : official publication of the Academy of Dental Materials. 1997 Jul;13)4(:258-69. PubMed PMID: 11696906.

O’Neill JF. Characterisation and susceptibility to leathal photosensitisation of in vitro-grown sub-gingival biofilms.: University College London; 2006.

Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture-base materials with different surface finishes. Journal of dentistry. 1998 Sep;26)7(:577-83. PubMed PMID: 9754746.

Bertazzoni Minelli E, Della Bora T, Benini A. Different microbial biofilm formation on polymethylmethacrylate )PMMA( bone cement loaded with gentamicin and vancomycin. Anaerobe. 2011 Dec;17)6(:380-3. PubMed PMID: 21515396.

Crick CR, Parkin IP. Preparation and characterisation of super-hydrophobic surfaces. Chemistry. 2010 Mar 22;16)12(:3568-88. PubMed PMID: 20209527.

Knorr SD, Combe EC, Wolff LF, Hodges JS. The surface free energy of dental gold-based materials. Dental materials : official publication of the Academy of Dental Materials. 2005 Mar;21)3(:272-7. PubMed PMID: 15705434.

Charman KM, Fernandez P, Loewy Z, Middleton AM. Attachment of Streptococcus oralis on acrylic substrates of varying roughness. Letters in applied microbiology. 2009 Apr;48)4(:472-7. PubMed PMID: 19228292.




DOI: http://dx.doi.org/10.26477/idj.v38i3.98

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